The most annoying mistake is over-playing, I played with few bitcents and reached really good profit but kept going for more until I lost the deposited and profit money. I think over-trading in general is a big mistake that can even lower the possibility more than the house edge.
You should know your limit and don't do that usually because it will just make you piss when you will lose a lot of money. It's not over trading but it is over gambling, you are just not aware of what can happen when you will not stop on the right time. Also control yourself when you gamble, so that you will not be losing your deposited and profit money.
If you have a really difficult time to do that, it might be better to just try to stop gambling alltogether, some people just lack the self-conbtrol to gamble with moderation an they'll always just spend too much.
Other people can benefit from just setting a limit and not going over that limit when they play. I'm talking about a time and/or spending limit btw.
Self control and moderation is what every gambler is lacking of. And out of those things they really can't do the right thing and that is leading most of us into doing a very wrong mistake or worst comes worst the unexpected mistake/s. That will be the only way to prevent ourselves to get through those mistakes by limiting ourselves.