1000w PSU is pretty borderline for mining with 6 card rig, to be honest (Always give yourself headroom with PSU. Efficiency is better and You can have a nice night sleep knowing that You aren't pushing Your PSU too hard)
Try GEN2 on Your PCI-E lanes.
That is very true!! and yes I tried Gen 2 a still problems with the 3rd GPU

I really don't know what else to try, I completed another fresh install of windows thinking it would be maybe a reg key that had got corrupt but yeah didn't work..
Debating to buy 3 more PCI risers and possibly RMA the motherboard for a new one or different one..
I've tried to reach out to Asrock but they haven't got back to me yet. Its so frustrating because this motherboard is meant to be a mining board, I wasn't expecting any of these problems...