GREAT NEWS! I've got it working!! 3 GPU's running successfully, wow cant thank you all enough for the help.

Russel I literally done what you said, went onto device manager and sorted by connection and disabled the audio adaptor to the GPU that was flagged with the yellow exclamation mark and then BOOM it worked. I can finally relax... And now look to get 3 more GPU's added to the works.. Thanks again to everyone to helped