So I can remove d=128 due to litecoin pool
Yes, in fact it is recommended. On litecoinpool.org.
and I can turn the wheel also if the usb stick is attached?
You mean the potmeter! No that's not recommended, unless you have good access and know what you are doing. You need to be seriously careful when fiddling with the potmeters while the Moonlander is powered: if the screwdriver slips and creates a short on the circuitboard it may end as goodbye Moonlander. If accidentally you turn the potmeter too much you may fry the ASIC or damage the potmeter (see page 1). So better not do it, just unplug and take it easy.