I have 2x 49 port hubs running they run 41 and 44 hubs each and I am running a 20 port hub.
one of my 49 port hubs has a bad row of 7 it has power but no hubs can be read on it. it is okay as I am using a few of those 7 dead ports to power usb 3 fans.
I think a good mobo can do 3x 49 port hubs and up to 120 sticks 40 on each. I have a third hub on the way.