The opportunity to play in the Europa League is still there because Roma, Florentina and Atalanta are still there but it depends on the result of the match from Lazio and these three teams. These three teams collect the same points, yes, 59 and have a difference of 3 points from Lazio. Lazio itself still has tough enough opponents, namely Juventus and Verona, who will certainly find it difficult to get 3 points.
If Lazio lose to Juventus in their next match, then the strongest candidate to occupy the 5th position in Serie A is AS Roma. I think like that because AS Roma will face a weak team, namely Venice, currently Venice is at the bottom of the table and they will lack the motivation to play against a strong team like Roma. Meanwhile, Atalanta and Fiorentina will face a pretty strong opponent.
If Lazio really loses points from Juventus, it will be very exciting to see the competition for 5th place. Four teams will fight for a ticket to the Europa League, Roma will benefit slightly because they have an easier opponent. Lazio will fight for their fate at Juventus house, in fact Juventus has no burden in the match against Lazio, because their position is safe in 4th place, but Juventus needs a win to finish in 3rd place.