Will Rudiger still play against Barcelona ?
He was injured badly against the Ukrainian side
I didn't watch bit saw the video how bad could it be

Rudiger's goal in the match against Shakhtar Donestk did make the position a draw and Madrid avoided defeat but indeed this had to be paid dearly because the collision with Anatoly Trubin made his head have to be stitched with 20 stitches and there is still no statement about this but there are some reports that Rudiger is doubtful play in the next game.
I actually think the opposite, Barcelona have failed miserably on the UCL stage after being defeated by Inter Milan in the first leg in a draw in the second leg which was played earlier this morning and that made their chance to qualify for the round of 16 almost of course The mentality of the Barcelona players is not good.
and that is in contrast to the Real Madrid team which is very stable at the moment.
El CLasico is always interesting and of course played with high tension and if you look at the consistency of the two teams at this time I believe Real Madrid will be able to win over Barcelona and I will bet big on their victory in this match.
Their points are 3 points behind Inter as the strongest competitor and to qualify is obviously very difficult now because indeed they must at least win in the last 2 matches against Plzen and Bayern but Inter are definitely bigger in chances because for them there is still Plzen that can be used as a stepping stone but for Bayern it is definitely not an easy opponent to beat, especially for them (Barcelona) already feel that against Bayern it is difficult even to dominate.