in addition to the options for losing keys mentioned by Biodom, there is always an unexpected hacking option. We can recall the story that happened a few months ago with Libbitcoin, where key generation was carried out with insufficient entropy, as a result of which the keys generated in this way were especially vulnerable to hackers. This way, you can be confident in the safety of your keys and still face the loss of your bitcoins. The probability of this is almost zero, but still not zero.