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Empoli qualified for the semi-finals, perhaps because of their luck, which led to a penalty shootout victory. Both teams attacked well against Juventus from start to finish, but neither could score a goal, so the match ended in a draw, which led to a thriller in which Empoli won. Yes, Juventus tried hard to put themselves in a good position in front of their fans, but they failed to attack enough. However, Empoli have made their efforts bear fruit and have qualified for the semi-finals.
This is huge in both ways. For Juventus it is one of the worst defeats they suffered this season and for Empoli, if they can get away from the relegation zone somehow, this win would be saving their season for the better. Of course the Coppa Italia wouldn't make up for getting relegated into Serie B, but this win can give them some positive energy for all the games to come as they have something great to look forward to.
For Juventus on the other hand, losing this game sends a message to all the other teams that effort does pay off even when they play at home. Since they are probably safely in the top five, the win of the Coppa Italia wouldn't have given them any advantage with respect to qualification for other competitions, but still this is a huge blow to what they have planned for this season.