No, you don't lose it. The casino could recover your funds if you ask them to, although you should insist that they do so, because it is your own mistake if you do so. It is your responsibility to check before making any deposit at the casino, but I don't think the casino will refuse to recover your funds if you request it in the correct way, although it could take a while to do so, so you would have to be very patient.
I doubt that a cryptocurrency deposit sent to a gambling account on the wrong network is easy to be recovered because it is supposed to be assumed lost or inaccessible to the gambling site, making it very difficult, if not next to impossible.
Even after taking necessary actions to immediately contact the cryptocurrency gambling site's customer support and even provide them with the transaction hash ID, it might still be impossible to recover such funds. So it's best to triple check before making any such transactions into a gambling site wallet.